σ B contributes to Listeria monocytogenes invasion by controlling expression of inlA and inlB
نویسندگان
چکیده
منابع مشابه
The inlA gene required for cell invasion is conserved and specific to Listeria monocytogenes.
The Gram-positive bacterium Listeria monocytogenes can actively induce its own uptake by epithelial cells and fibroblasts through a surface-exposed 80 kDa protein, internalin (InIA), encoded by inIA. We studied the distribution and the DNA polymorphism of inIA sequences in a wide variety of wild strains of L. monocytogenes as compared to other Listeria species. This was done by PCR-amplifying i...
متن کاملListeria monocytogenes B Contributes to Invasion of Human Intestinal Epithelial Cells
The gram-positive facultative intracellular food-borne pathogen Listeria monocytogenes is associated with serious invasive infections in humans and animals (10). To establish a foodborne bacterial infection, a pathogen must survive rapidly changing environmental conditions encountered in the gastrointestinal tract, such as exposure to bile salts, organic acids, and changing osmolarity. Survival...
متن کاملSigma B contributes to PrfA-mediated virulence in Listeria monocytogenes.
Transcription of the Listeria monocytogenes positive regulatory factor A protein (PrfA) is initiated from either of two promoters immediately upstream of prfA (prfAp(1) and prfAp(2)) or from the upstream plcA promoter. We demonstrate that prfAp(2) is a functional sigma(B)-dependent promoter and that a sigB deletion mutation affects the virulence phenotype of L. monocytogenes. Thus, the alternat...
متن کاملThe Listeria monocytogenes σB Regulon and Its Virulence-Associated Functions Are Inhibited by a Small Molecule
UNLABELLED The stress-responsive alternative sigma factor σ(B) is conserved across diverse Gram-positive bacterial genera. In Listeria monocytogenes, σ(B) regulates transcription of >150 genes, including genes contributing to virulence and to bacterial survival under host-associated stress conditions, such as those encountered in the human gastrointestinal lumen. An inhibitor of L. monocytogene...
متن کاملListeria monocytogenes sigmaB contributes to invasion of human intestinal epithelial cells.
The role of sigma(B) in Listeria monocytogenes infection of human intestinal epithelial cells was investigated. Invasion defects associated with loss of sigma(B) paralleled those of a DeltainlA strain independently of the sigma(B)-dependent P2(prfA) promoter. Concomitantly, amounts of inlA transcript and InlA protein were significantly decreased in the DeltasigB strain.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbiology
سال: 2005
ISSN: 1350-0872,1465-2080
DOI: 10.1099/mic.0.28070-0